Worked example Prediction - Test

1. Log in into HiPathia. For further information on this step visit Logging in.

2. We will test the model with another Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix of the test data from the link:

• Test expression matrix: brca_genes_vals_bn_test.txt

3. Upload the data to HiPathia in the data panel by clicking on My data. For further information on this step visit Upload your data.

4. Press the Prediction button.

5. In the Type panel, select Test existing predictor. A window with all the existing models will appear. Select the model you want to use. We will use the model we have created in Worked example Prediction - Train. The model information will appear on the right panel.

6. In the Input data panel select Expression matrix. Press the File browser of the Expression matrix file, and select the desired file, brca_genes_vals_bn_test.txt.

7. In the Job information panel, press the File browser button and select the desired output folder. In this case we will use analysis_BRCA. Give a name to the job, for example “BRCA test model”.

8. Press the Run analysis button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the My jobs button.