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perturbation_effect [2020/01/27 21:15] krianperturbation_effect [2021/01/26 16:10] (current) – update old results example ialamo
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 The **Perturbation effect module** can be accessed from the main menu bar, by clicking on the //Perturbation effect// button. The **Perturbation effect module** can be accessed from the main menu bar, by clicking on the //Perturbation effect// button.
 {{ :perturbation_effect_form.png?nolink |}} {{ :perturbation_effect_form.png?nolink |}}
- ===== INPUT =====  +===== Perturbation effect form ===== 
- +The main page of the tool is a form. This form includes all the information and parameters needed to process a perturbation effect study.  
-The main page of the tool is a form. This form includes all the information and parameters needed to process a perturbation effect study. The form is divided in different panels:+{{ ::perturbationeffectform.png?nolink |}} 
 +The form is divided in different panels:
 ==== Expression matrix file ===== ==== Expression matrix file =====
   * **[[Expression matrix file format |Expression matrix file]]** : Expression matrix provided by ourselves (see how to upload files in [[upload_your_data|Upload your data]]).   * **[[Expression matrix file format |Expression matrix file]]** : Expression matrix provided by ourselves (see how to upload files in [[upload_your_data|Upload your data]]).
 +{{ ::expmatrfile.png?nolink |}} 
 +When we select a gene expression file, the number of samples of this matrix will appear under the “File browser” button as shown below. 
 +{{ ::pertnumberofsamples.png?nolink |}}
 ==== Sample ==== ==== Sample ====
  
 Once the file is selected the column names of the expression matrix will be loaded in this panel, we have to select the column to proceed.  Once the file is selected the column names of the expression matrix will be loaded in this panel, we have to select the column to proceed. 
-{{ :persurbationeffect.png |}}+{{ ::samplesperteffectform.png?nolink |}}
  
 ==== Study information ==== ==== Study information ====
-For further information about this panel, see [[job information|Study information ]]+This panel includes some parameters in order to identify and save our study.  
 +  * **Output folder**: If we want to reorganize our studies we can select the folder in which we want save our report. By default the study will be saved in the home in a folder named "Perturbation_effect_study-N"N is an integer number. 
 +  * **Study name**: We can give a name to our study. This is very useful to later identify it among the other studies listed in the //My studies// list.\\ The default study name is "Perturbation_effect_study-N", N is an integer number. 
 +  * **Description**: We can give a description to our study. 
 +{{ ::studyinfopereffectform.png?nolink |}} 
 +==== Run analysis ==== 
 +Once the form has been filled in, press the //Run analysis// button to launch the study. Your study will be listed in the //My studies// panel. 
 +{{ ::runanalysis.png?nolink |}} 
 +Your study will be listed in the //My studies// panel, and a panel called //Browse my studies// will appear showing all your studies and their state. the new study will appear with a **queued** state then **running** state. If everything goes well, the state will be **done** after few minutes(depending on the inputs data and the availability of server). 
 +All study states are:  
 +  * **Queued**: The information has been processed and the study has been sent and waits to be processed. 
 +{{ ::queuedpereff.png?nolink |}} 
 +  * **Running**: The study is in progress, study can be cancelled using the stop button. 
 +{{ ::runingperteffect.png?nolink |}} 
 +  * **Done**: The study has ended and the results are available to visualize and download. 
 +  * **Cancelled**: The study was canceled before finishing. 
 +  * **Error**: Sometimes a study can stop returning a error message, you can report and contact us in order to help you to fix it. 
 +{{ ::studiesperteffect.png?nolink |}}
  
-Once the form has been filled in, press the //Run analysis// button to launch the study. Your study will be listed in the //My studies// panel. 
  
-===== OUTPUT =====+===== Perturbation effect report =====
  
 The results page of the Perturbation effect tool is an ** interactive working environment **. You can **simulate knock outs or over-expressions of one or several genes ** or the ** effect of a drug** in the activity of signaling pathways. The results page of the Perturbation effect tool is an ** interactive working environment **. You can **simulate knock outs or over-expressions of one or several genes ** or the ** effect of a drug** in the activity of signaling pathways.
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 Last update gene list Last update gene list
  
-==== Interpretation of the results ==== +==== Worked example and Interpretation of the results ==== 
- For further information about the interpretation of the results, see [[Perturbation effect results|Perturbation effect results]]. +You can find an example of the use of this tool and the interpretation of the results at [[worked_example_perturbation_effect|Worked example: Perturbation effect]].
- +
perturbation_effect.1580159707.txt.gz · Last modified: 2020/01/27 21:15 by krian