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worked_example_differential_example [2021/01/14 13:41] – [Worked example Differential signaling] krianworked_example_differential_example [2021/01/28 21:23] (current) krian
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 ===== The oestrogen receptor (ER+) positive group Vs negative group (ER-) ===== ===== The oestrogen receptor (ER+) positive group Vs negative group (ER-) =====
 ==== Differential signaling inputs ==== ==== Differential signaling inputs ====
 +**Note:** You can also launch the Differential signaling example by clicking the //Run example// button or the //help// button on the header and then //Differential signaling example//.
 +
 **1-** Log into HiPathia. For further information on this step visit [[logging_in|logging in]]. **1-** Log into HiPathia. For further information on this step visit [[logging_in|logging in]].
  
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   * Experimental design:[[http://hipathia.babelomics.org/data/brca_designmatrix_ERposneg.tsv|brca_designmatrix_ERposneg.tsv]]   * Experimental design:[[http://hipathia.babelomics.org/data/brca_designmatrix_ERposneg.tsv|brca_designmatrix_ERposneg.tsv]]
  
-**3-** Upload the normalized data to HiPathia by clicking //My data// in the data panel. For further information on this step visit [[upload_your_data|Upload your data]].+**3-** Upload the normalized data to HiPathia by clicking //My data// in the data panel, or click the //run example// button. For further information on this step visit [[upload_your_data|Upload your data]].
  
 **4-** Click //Differential signaling// button.  **4-** Click //Differential signaling// button. 
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 {{ :hipathia_work1_study-browser.png?600 |}} {{ :hipathia_work1_study-browser.png?600 |}}
  
-==== Differential signaling results ====+This a video tutorial for differential signaling study: {{:youtube.png?direct&30|https://www.youtube.com/watch?v=ytyagfQpQPk&feature=youtu.be}}:https://www.youtube.com/watch?v=ytyagfQpQPk&feature=youtu.be 
 + 
 +==== Differential signaling results and interpretations ====
 Once the analysis is finished at HiPathia web, the report/results will be available in “My studies”.\\ The results page of the Differential signaling tool includes different output results. You can download any table or image showed in the report page by clicking the name right before it.\\ You can also download the circuit activity values and function matrices by clicking //Circuit values//, //GO terms values// and //Uniprot keywords values// respectively. Once the analysis is finished at HiPathia web, the report/results will be available in “My studies”.\\ The results page of the Differential signaling tool includes different output results. You can download any table or image showed in the report page by clicking the name right before it.\\ You can also download the circuit activity values and function matrices by clicking //Circuit values//, //GO terms values// and //Uniprot keywords values// respectively.
  
 +For further information about the differential signaling report please visit [[differential_signaling#differential_signaling_report|Differential signaling results]]
 +/*
 Furthermore, the report can be downloaded to be visualized locally.  Furthermore, the report can be downloaded to be visualized locally. 
 {{ ::downloadreport.png?nolink |}} {{ ::downloadreport.png?nolink |}}
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     * **Fold Change**: is a measure describing how much the ** circuit activity** changes between conditions.[[https://en.wikipedia.org/wiki/Fold_change|reed more about Fold Change]]     * **Fold Change**: is a measure describing how much the ** circuit activity** changes between conditions.[[https://en.wikipedia.org/wiki/Fold_change|reed more about Fold Change]]
     * **logFC**: The logarithm to base 2 of calculated Fold change.     * **logFC**: The logarithm to base 2 of calculated Fold change.
 +*/
  
 +/*
  ==== Results interpretations ====  ==== Results interpretations ====
 +*/
  
 **Estrogen receptor (ER) pathway (hsa04915):** **Estrogen receptor (ER) pathway (hsa04915):**
worked_example_differential_example.1610631690.txt.gz · Last modified: 2021/01/14 13:41 by krian