Expression matrix file is a Tab-separated values file.

A tab-separated values (TSV) file is a simple text format for storing data in a tabular structure (e.g. database or spreadsheet data). Each record in the table is one line of the text file. Each field value of a record is separated from the next by a tab stop character. More about TSV...

This file has two columns if there is only one sample, and more than two columns if there are many samples. The first line is a header and must contain the sample names. The first column corresponds to genes, probes or proteins, and the following IDs are accepted:

• Ensembl gene
• HGNC symbol
• Entrez id
• Affy HG U133A probeset
• Affy HG U133B probeset
• Affy HG U133-PLUS-2 probeset
• Affy HTA 2.0

The next columns correspond to gene expression values in numeric format from each sample.

Here is an example of a file with only one example:

id	sampleName
1	0.3
2	1
3	0.73

And here is another example with more than one sample:

id	sample1	sample2	sample3
1	0.31	0.6	0.24
2	1	0.81	0.91
3	0.7	0.9	0.3
4	0.23	0.45	0.33

For a file example see brca_genes_vals_bn.txt

Note: If probe expression values are provided, these are recodified to gene expression values, obtained as the average value of all the probes mapping in the gene.

The web version of Hipathia is limited to a maximum of 500 samples per dataset. For bigger datasets please use the R Bioconductor Hipathia package